Role of oral phytogenic supplementation to protect cardiac, hepatic, nephrotic, and splenic oxidative stress in broiler chickens.

This study investigated the effects of adding essential oils of garlic, ginger, turmeric, and cinnamon to drinking water on cardiac, hepatic, nephrotic, and splenic oxidative status of broiler chickens. A batch of 200 1-d old Arbo acre broiler chicks was administered with Control (Water: no additive), 30 ml/L of cinnamon, ginger, turmeric, or garlic essential oils in drinking water for 42 d. On day 43, three broiler chickens/replicates were sampled randomly, sacrificed, and eviscerated. The hearts, spleens, kidneys, and livers were excised and assayed for glutathione peroxidase, total antioxidant activity, catalase, superoxide dismutase, and lipid peroxidation using standard protocols. In spleen broiler chickens, all additive essential oils increased (P < 0.05) total antioxidant activity. Catalase, superoxide dismutase, and glutathione peroxidase significantly increased (P < 0.05) in garlic, ginger, and turmeric essential oils except cinnamon. In kidney broiler chickens, lipid peroxidation was significantly reduced (P < 0.05) in all the additive essential oils. Garlic, cinnamon, and ginger essential oils increased (P < 0.05) catalase, superoxide dismutase, and glutathione peroxidase in kidney broiler chickens. In liver broiler chickens, lipid peroxidation, and glutathione peroxidase were higher (P < 0.05) in cinnamon essential oil than other additive essential oils. Superoxide dismutase and catalase were higher (P < 0.05) in turmeric essential oils. In heart broiler chickens, all the additive essential oils significantly decreased (P < 0.05) lipid peroxidation and increased (P < 0.05) total antioxidant activity. In conclusion, oral garlic, turmeric, and ginger essential oils supplementation did not reduce lipid peroxidation in spleen, whereas cinnamon essential oil caused lipid peroxidation in liver of broiler chickens.

Herbal supplements suppress pro-inflammatory cytokines, boost humoral immunity, and modulate adipokines to enhance the productivity traits of rabbit bucks in hot climatic conditions.

Thermal stress is the main stressor accounting for reduced productivity, compromised immunity, and collapse of thermoregulatory measures in rabbits in the tropics. The current climate change depicts worsening assault of heat stress in the time ahead; hence, the need to develop combative measures for animal productivity. This research investigates the influence of herbal supplements of three tropical herbs Viscum album (mistletoe), Moringa oleifera (Moringa), and Phyllanthus amarus (Phyllanthus) on immune response, oxidative status, adipokines, and growth of eighty weaned rabbits during heat stress in tropical climate. The bucks were fed with four standard diets; a control and others supplemented with each of Moringa, Phyllanthus, and mistletoe for an eight-week feed trial. Performance indicators were monitored and blood were sampled and assayed for hematology, pro-inflammatory cytokines, adipokines, and oxidative status. The result shows that the performance of bucks fed with Phyllanthus and mistletoe supplements was superior to other groups. The neutrophil/lymphocyte ratio was significantly (p < 0.05) lower in the bucks fed with Moringa supplement, with significantly (p < 0.05) highest values obtained in the control group. Total antioxidant activity of the bucks fed with supplements was significantly (p < 0.05) higher than those on control, with the significantly (p < 0.05) highest value recorded in bucks fed with Phyllanthus. Serum lipid peroxidation of the bucks on control was significantly (p < 0.05) highest and significantly (p < 0.05) least value was obtained in bucks on mistletoe. Heat shock protein 70, adiponectin, and leptin of the bucks on control were significantly (p < 0.05) higher than bucks on herbal supplements. Interleukin 6, interleukin ß, and tumor necrosis factor α of bucks on control were significantly (p < 0.05) higher than bucks fed on herbal supplements. In conclusion, the inclusion of herbal supplements Moringa, Phyllanthus, or mistletoe suppressed pro-inflammatory cytokines, boost humoral immunity, enhance the anti-oxidative status, and promote the growth of rabbit bucks during thermal discomfort.